You are here: Home / News / Cell-Specific Information Processing in Segregating Cell Populations

Cell-Specific Information Processing in Segregating Cell Populations

In this Research Article published in Science, we provide the first systems- and cell-specific network models of contact-initiated signaling between two distinct cell types.

Eph network

 

Cells have self-organizing properties that control their behavior in complex tissues. Contact between cells expressing either B-type Eph receptors or their transmembrane ephrin ligands initiates bidirectional signals that regulate cell positioning. However, simultaneously investigating how information is processed in two interacting cell types remains a challenge. We implemented a proteomic strategy to systematically determine cell-specific signaling networks underlying EphB2- and ephrin-B1–controlled cell sorting. Quantitative mass spectrometric analysis of mixed populations of EphB2- and ephrin-B1–expressing cells that were labeled with different isotopes revealed cellspecific tyrosine phosphorylation events. Functional associations between these phosphotyrosine signaling networks and cell sorting were established with small interfering RNA screening. Data-driven network modeling revealed that signaling between mixed EphB2- and ephrin-B1–expressing cells is asymmetric, and that the distinct cell types use different tyrosine kinases and targets to process signals induced by cell-cell contact. We provide systems- and cell-specific network models of
contact-initiated signaling between two distinct cell types.


Jørgensen et al., Science December 11th 2009.

Document Actions

Filed under: , ,
« June 2017 »
June
SuMoTuWeThFrSa
123
45678910
11121314151617
18192021222324
252627282930